Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation
Moreno-García, Jaime; Mauricio, Juan Carlos; Moreno, Juan; García-Martínez, Teresa
Several Saccharomyces cerevisiae strains (flor yeasts) form a biofilm (flor velum) on the
surface of Sherry wines after fermentation, when glucose is depleted. This flor velum is fundamental
to biological aging of these particular wines. In this study, we identify abundant proteins in the
formation of the biofilm of an industrial flor yeast strain. A database search to enrich flor yeast“biological process” and “cellular component” according to Gene Ontology Terminology (GO Terms)
and, “pathways” was carried out. The most abundant proteins detected were largely involved
in respiration, translation, stress damage prevention and repair, amino acid metabolism (glycine,
isoleucine, leucine and arginine), glycolysis/gluconeogenesis and biosynthesis of vitamin B9 (folate).
These proteins were located in cellular components as in the peroxisome, mitochondria, vacuole, cell
wall and extracellular region; being these two last directly related with the flor formation. Proteins
like Bgl2p, Gcv3p, Hyp2p, Mdh1p, Suc2p and Ygp1p were quantified in very high levels. This study
reveals some expected processes and provides new and important information for the design of
conditions and genetic constructions of flor yeasts for improving the cellular survival and, thus,
to optimize biological aging of Sherry wine production
↧